✦ Volcano Plot Generator

Volcano Plot Generator
for RNA-seq & Proteomics

Generate publication-ready volcano plots from RNA-seq, proteomics, and GWAS data. Custom thresholds, gene labels, and color schemes — no R or Python required.

Get Started for Free →
Free daily creditsEditable SVG exportPublication-ready output

Try a sample dataset or upload your own

or upload your own

Upload differential expression data

Drag and drop your DESeq2, edgeR, limma, proteomics, or GWAS file here, or click to choose a file.

CSV, TSV, TXT, Excel · Max 10 MB
Fig. 01Examples

Volcano plot examples generated from research data

What researchers uploaded → what FigCanvas generated.

Immunology
Volcano plot of T cell differential expression in irradiated vs untreated tumor
Phosphoproteomics
Volcano plot of GCN2-KO phosphoproteomics UV treatment analysis
Survival Analysis
Volcano plot of hazard ratio survival analysis with gene annotations
Proteomics
Volcano plot of proteomics fold change highlighting significant proteins
Drug Response
Volcano plot of RSL3 vs control differential expression analysis
RNA-seq
Volcano plot of RNA-seq gene expression with up and downregulated genes
Transcription Factor
Volcano plot of ALX1 loss showing transcription factor target changes
Cytokine Signaling
Volcano plot of cytokine signaling pathway differential expression
§ Coverage

Volcano plot types FigCanvas can generate

From RNA-seq differential expression to GWAS results, FigCanvas handles the full range of volcano plot types used in research publications.

— RNA-seq

RNA-seq Volcano Plots

Visualize differential expression results from DESeq2, edgeR, or limma. Annotate key genes, set custom fold change and FDR thresholds.

— Proteomics

Proteomics Volcano Plots

Create volcano plots from SILAC, TMT, or label-free quantification data. Label top hits by significance, pathway, or custom gene list.

— GWAS

GWAS Visualization

Plot genome-wide association results with significance thresholds, SNP annotations, and chromosome-based color coding.

— Metabolomics

Metabolomics Volcano Plots

Generate volcano plots from metabolomics datasets with pathway-level annotations and support for positive and negative ion mode data.

— Multi-condition

Multi-condition Comparisons

Compare differentially expressed features across multiple conditions or timepoints with consistent formatting and shared axis scales.

— Custom

Custom Threshold and Label Plots

Set custom significance cutoffs, highlight specific genes or proteins by name, and choose color schemes suited for publication and accessibility.

§ MethodFour steps

How FigCanvas works

Go from differential expression data to a publication-ready volcano plot in four steps.

Step 01

Upload differential expression data

Upload your RNA-seq, proteomics, or other differential analysis results in CSV or Excel format. FigCanvas auto-detects key columns such as log2 fold change and adjusted p-value.

Step 02

Analyze and recommend a setup

FigCanvas inspects your data structure and identifies the most suitable volcano plot workflow, including thresholds, labeling strategy, and visual emphasis.

Step 03

Generate a publication-ready plot

Using research-focused visual defaults, FigCanvas creates a clean volcano plot with balanced color, clear thresholds, and journal-style presentation.

Step 04

Refine, vectorize, and export

Fine-tune labels and styling, convert the figure into an editable vector graphic if needed, and export as SVG, PDF, or PNG.

Fig. 04Why FigCanvas

Why FigCanvas for publication-ready volcano plots

01

No R or Python required

Skip the ggplot2 setup, package installation, and debugging. Upload your data and FigCanvas generates the volcano plot directly — the same quality you would get from EnhancedVolcano, without a single line of code.

02

Publication-ready output by default

Default styling follows common journal figure conventions, with clean backgrounds, balanced color emphasis, clear significance thresholds, and readable axis labeling.

03

Smart gene label positioning

Annotate specific genes and let FigCanvas place labels automatically to reduce overlap and improve readability in dense volcano plots.

04

Flexible export for papers, posters, slides

Export your volcano plot as SVG, PNG, or PDF for manuscripts, posters, presentations, and journal submission workflows. SVG output also makes it easier to refine the figure in vector editing tools.

Fig. 05Workflows

Volcano plots for research workflows

Use FigCanvas to create volcano plots for differential expression analysis, publication figures, posters, and scientific presentations.

For RNA-seq studies

Create volcano plots from DESeq2, edgeR, or limma results. Highlight significant genes, apply fold change and FDR thresholds, and prepare figures for differential expression analysis.

For proteomics and metabolomics

Generate volcano plots from differential protein or metabolite analysis, annotate top hits, and visualize significance with publication-ready styling.

For multi-omics and comparative analysis

Use volcano plots to compare conditions, highlight significant features, and visualize differential signals across multiple omics workflows.

For manuscripts and posters

Export publication-ready volcano plots for journal figures, conference posters, slides, and other research communication workflows.

§ Q & A6 entries

Volcano Plot Generator FAQs

A volcano plot is a scatter plot used in genomics and proteomics to visualize differential expression results. The x-axis shows log2 fold change and the y-axis shows -log10 adjusted p-value. Significantly differentially expressed genes appear in the upper corners, forming a volcano shape.

FigCanvas accepts CSV or Excel files with columns for gene name, log2FoldChange (or logFC), and adjusted p-value (padj, adj.P.Val, or FDR). Output from DESeq2, edgeR, limma, and most other differential expression tools is supported directly.

In R (ggplot2, EnhancedVolcano) or Python (matplotlib), you write code, debug it, and format the plot manually — typically taking 30–60 minutes. FigCanvas generates the same quality plot in under 60 seconds with zero code. You can then edit colors, labels, and thresholds interactively.

Yes. You can specify a list of gene names to label directly on the plot. FigCanvas automatically positions labels to avoid overlap, following the same conventions used in EnhancedVolcano and ggrepel.

Yes. FigCanvas exports at 300 DPI with standard publication fonts. The default aesthetic matches what you would see in a Nature or Cell paper. SVG output is fully editable in Adobe Illustrator or Inkscape.

Yes. FigCanvas supports volcano plots for proteomics (SILAC, TMT, LFQ), GWAS results, metabolomics, and other datasets that produce fold change and significance values.

§ Start

Generate publication-ready volcano plots from your data

Upload DESeq2, edgeR, or limma results and create volcano plots with gene labels, significance thresholds, and journal-ready styling — no coding required.

Free daily creditsEditable SVG exportPublication-ready output
Try the volcano plot generator— it's free